Dna gel electrophorosis essays

Dna gel electrophorosis essays DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic acid molecule which determines inherited structure of a protein. The steps are made of bases: adenine, guanine, cytosine, and thymine. The sides are sugar and phosphate molecules. Restriction enzymes are enzymes that cut DNA at restriction sites, leaving fragments blunt or sticky. The restriction fragments are separated using a technique called DNA has a negative charge so when an electrical charge is applied it makes DNA move to the positive side. DNA is placed in agarose gel. Smaller fragments move faster. The purpose of this lab is to separate DNA fragments using gel electrophoresis. Hind III cuts AAGCTT between the two irst As. EcoRI cuts at GAATTC between the G and the A. Hind III and EcoRI both make sticky ends. Our results for this lab were EcoRI separated into five fragments. Hind III separated into four fragments. The control only had one fragment. (See chart A and figure 1-1 for distances) The purpose of this lab was to see how gel electrophoresis separates DNA fragments. We used Hind III, EcoRI, and a controlled enzyme. Some fragments were hard to see because of smearing. These were the bigger fragments. Loading the DNA was difficult and if you werent careful you could rupture the wells which ruined the lab. We, fortunately, did not run into this problem. ...